Monoethanolamine Treatment of Fish Wastes and Salmon Guts to Increase It Palmitoylethanolamide and Anandamide Contents

This study was carried out to determine the palmitoylethanolamide (PEA) and arachidonoylethanolamide (AEA) or anandamide contents in selected fish wastes, treating wastes with highest PEA AEA different concentration of monoethanolamine (MEA) solution, incubation temperature time, as well ratio MEA solution waste further increase its contents.
 Based on results preliminary experiment, a fractional factorial design experiments done 4 factors including concentration, dosing (MEA solution:salmon guts). The showed that content ranged from 2.25 8.06 mg/g sample, 17.4 300.2 µg/g sample while 1.3 19.0 all wet weight basis FD treated samples. MEA, using an 250mM pure chemical, time 0.5 hour, 6oC dose 6 mL solution:100 g salmon guts. samples were analysed Yates algorithm which four more important. significantly affected by used guts, followed then slight effect has no significant effect.